Project
RNA II (A)
Part:BBa_K2259000:Design
Designed by: Laurynas Karpus Group: iGEM17_Vilnius-Lithuania (2017-10-01)
SynORI framework RNA II - Replication Initiator (Group A)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design
Sequence has been mutated to inactivate RNR 1 from the anti-sense strand.
Having the knowledge on ColE1 replication,It immediately becomes clear that in order to control the copy number of a plasmid one could simple change RNA I promoter. But there is a reason it’s never done before!
<PAPILDYTI>
Source
Wildtype ColE1 sequence was taken from addgene, mutated and ordered from IDT.