Project
RNA II (A)

Part:BBa_K2259000:Design

Designed by: Laurynas Karpus   Group: iGEM17_Vilnius-Lithuania   (2017-10-01)


SynORI framework RNA II - Replication Initiator (Group A)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design

Sequence has been mutated to inactivate RNR 1 from the anti-sense strand.

Having the knowledge on ColE1 replication,It immediately becomes clear that in order to control the copy number of a plasmid one could simple change RNA I promoter. But there is a reason it’s never done before!

<PAPILDYTI>

Source

Wildtype ColE1 sequence was taken from addgene, mutated and ordered from IDT.

References